Transfection Reagents
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM siRNA Transf
Short interfering RNA (siRNA) can be directlyintroduced intocells by transfection. A lipid-based transfection reagent such as X-tremeGENE siRNA Transfection reagent can provide a convenient reliable and efficient vehicle for delivering siRNAs into animal cells enabling the study of cellular and functional consequences of gene knockdown.This innovative reagent forms a complex with siRNA as well as with mixtures of siRNA and plasmid DNA (cotransfection) and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps mix and incubate diluted transfection reagent with diluted siRNA then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity it can be used without media changes. The product is animal-component free.ContentsSOLUTIONon filtered through 0.2 mum pore size membrane supplied in polypropy
Agilent Technologies BL21(DE3) PLYSS COMP CELLS
BL21(DE3) pLysS Competent Cell show high-level protein expression, codes for T7 lysozyme and a T7 RNA polymerase inhibitor,provide tighter control of protein expression for expression of toxic proteins,can also be used for non-toxic protien
Agilent Technologies GIGAPACK III XL PACK EXTRACT
Gigapack III XL packaging extract is used to package recombinant lambda phage with selectivity for phage containing large inserts. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries
Agilent Technologies XL1-BLUE ELECTROCOMP CELLS
XL1-Blue Electroporation-Competent Cells are optimal hosts for propagating both lambda and plasmid vectors. They are endA minus cells, Improve the quality of miniprep DNA, allow for color screening and single-strand rescue of phagemid DNA
Agilent Technologies XL1-BLUE SUPERCOMPETENT CELLS
Cell, Supercompetent, XL1-Blue; Stratagene; For high-efficiency cloning, greater than equal1 x 109 transformant/ug; Preparation of quality plasmid DNA; Blue-white color screening; Contain antibiotic-resistant F episome; Versatile cloning
Agilent Technologies SURE COMPETENT CELLS
SURE Competent Cells. For routine cloning of DNA with secondary structures, > /= 5 x 108 transformants/microgram. Restriction-Minus Competent Cells for Most Eukaryotic DNA. F- derivative for BAC cloning.
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit L
The SE Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL SE Cell Line Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessel12 x Single use pipettes
Agilent Technologies ELECTROTEN-BLUE E.COMP CELLS
ElectroTen-Blue Electroporation-Competent Cells 5 x 0.1mL and pUC18 control plasmid Highest electroporation efficiency (Hee) phenotype use for extrememly demanding cloning= 1 x 1010 transformants/microgram
Eurofins Discoverx KILR Retroparticles for Suspension Cells (Hygromycin B), 4 vials x 1.0. (97-0006)
The KILR Retroparticles for Suspension Cells (Hygromycin B) are MMLV particles designed to transduce suspension cells. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-cells-(hygr-97-0006.
Boca Scientific Inc NeuroMag Neuron Specific Transfection Reagent, 1ml
NeuroMag Transfection Reagent is a Magnetofection transfection reagent for neurons from 1 DIV to 21 DIV. NeuroMag has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. High transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells and other cell lines. With the unique properties of NeuroMag, transfected neurons can be followed for several days.
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.