Transfection Reagents
DIASYS DIAGNOSTIC SYSTEMS USA LLC DiaSys ASAT FS is a ready-to-use two component photometric reagent to determine Aspartate Aminotransferase in serum or heparin plasma on photometric systems. Each set contains R1=10L, R2=2.5L
Diagnostic reagent for quantitative in vitro determination of Aspartate Aminotransferase (ASAT, AST) in animal serum or heparin plasma on photometric systems. For veterinary or research use only. Increased ASAT, formerly called Glutamic Pyruvic Transaminase (GPT), levels may occur in connection with damages of heart or skeletal muscle as well as of liver parenchyma. Parallel measurement of ALAT and ASAT is recommended to distinguish liver from heart or skeletal muscle damages. Method: Optimized UV-test according to IFCC (International Federation of Clinical Chemistry and Laboratory Medicine). The reagent has a wide measuring range from 1.2 U/L to 600 U/L and very good precision. Reagent 1 contains TRIS, L-Aspartate Malate Dehydrogenase and Lactate Dehydrogenase, reagent 2 contains 2-Oxogluterate and NADH. Reagents must be stored refrigerated.
Takara Bio XFECTA PROTEIN TRANS RGNT 30PK
Xfect Protein is a novel, peptide-based transfection reagent that efficiently delivers a large amount of biologically active protein directly into live cells, with low cytotoxicity.
Takara Bio Xfect™ RNA Transfection Reagent | 1.2mL
Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA, including messenger RNA (mRNA), small interfering RNA (siRNA), and single guide RNA (sgRNA).The protocol is simple, and transfections can be carried out entirely in the presence of serum. Xfect RNA Transfection Reagent creates biodegradable nanoparticles when combined with RNA, resulting in very low cytotoxicity.
Lonza Walkersville SF Cell Line 4D-Nucleofector LV Kit XL, Catalog Number V4LC-2002, Reaction Volume Up to 20 mL, Kit Size 5 reactions, Processing Time Less than 10 min, Compatible System 4D-Nucleofector LV Unit
The Lonza SF Cell Line 4D-Nucleofector LV Kit XL is specifically designed for efficient, large-scale transfection of hard-to-transfect cell lines using the 4D-Nucleofector LV Unit. This closed-system nucleofection kit modifies up to 1 x 10^9 cells effectively, retaining cell viability. It allows variable reaction volumes and rapid processing, making it ideal for transient protein production and generating modified cell lines for various assays.
- Compatible system: 4D-Nucleofector LV Unit
- Reaction volume: Up to 20 mL
- Cell numbers supported: Up to 1 x 10^9 cells
- Processing time: Less than 10 minutes
- Kit size: 5 reactions
- Optimal cell lines: HEK293 suspension clones, CHO-S suspension clones, K-562, U-937
- Applications: Large scale transient protein production, generation of modified cell lines
- Key benefits: High transfection efficiencies, maintained cell functionality
- Storage conditions: 2 to 8 °C
Apexbio Technology LLC Calcium Phosphate Cell Transfection Kit 200T
Calcium phosphate transfection is a commonly used method for introducing exogenous DNA into eukaryotic cells DNA-calcium phosphate precipitate adheres to cells by interacting with their cell surface The exogenous DNA is subsequently internalized via cellular endocytosis ultimately delivering the DNA into target cells This method has been successfully applied to various cell lines to achieve transient or stable transfections HEK293 cells are most suitable for calcium phosphate transfection Hela and CHO cells can also be transfected using the calcium phosphate method with slightly lower transfection efficiency This product significantly enhancing transfection efficiency and reducing cytotoxicity Additionally the kit provides highly consistent pH values and salt concentrations ensuring reliable transfection efficiency across different experiments and meet for the transfection needs of most adherent and suspension cells
Eurofins Discoverx KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B), 2 vials x 0.5 (Adherent), 2 vials x 1.0 (Suspension). (97-0008)
The KILR Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of ProLabel-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology. The retroparticles mix can be used to transduce target cells with a ProLabel-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release ProLabel to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/kilr/retroparticles-for-suspension-adherent-c-97-0008.
Active Motif Chariot™
Chariot is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
Andwin Scientific TRANSFECTION REAGENT + BUFFER
The jetPRIME™ DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 188 reactions in 6-well plates and has a buffer volume of 60 mL. It is sold individually.